Extracellular vesicles derived from mesenchymal stem/stromal cells derived from dental pulp of exfoliated teeth induce osteogenic differentiation.

摘要: INTRODUCTION: Large bone defects in the field of orthopedic surgery remains challenging, primarily due to restricted regenerative capacity of bone tissue. The biomimetic bone implants could improve the osteoregeneration process, but the optimal composition for biomimetics remains unknown. Considering the great potential of extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) in regenerative medicine, our aim was to investigate the osteoinductive potential of EVs derived from MSCs from the dental pulp of human exfoliated deciduous teeth (SHEDs) either undifferentiated or those undergone differentiation into osteoblasts (osteoSHED-EVs), for potential use in biomimetic bone implants. EXPERIMENTAL: SHEDs were isolated from the dental pulp of 5 different donors of deciduous teeth and subjected to characterization by Flow cytometry (FCM). EVs-SHED and EVs-osteoSHED …