Age related RGC vulnerability in an autophagy deficient mouse model

摘要: Purpose: Glaucoma is an age-related neurodegenerative disease and one of the world’s leading causes for blindness. In this study we analyse the age related RGC susceptibility in context of autophagy impairment using Ambra1+/gt mice. Ambra1 is involved in autophagy induction, but also mitophagy. We also aimed to understand differences between WT and HT animals after ONC in greater detail.Methods: Optic nerve crush was performed in 4 and 13-month-old Ambra1+/gt HT mice and Ambra1+/+ WT controls. RGCS were stained with Gamma-synuclein and Brn3a in flatmounts and cryosections. RGC counts were performed 7days after ONC. qPCR analysis of oxidative stress response markers and mitophagy receptors in the retina was performed 3 and 7 days post-ONC and control eyes. Proteomic analysis of HT and WT retinae was performed using an ESI-Orbitrap-MS system.Results: Young Ambra1+/gt (HT …