摘要: This chapter presents techniques for the generation of mitotic chromosomes in vitro using Xenopus egg extracts. Discussions of technical details required for the successful use of the system have been included. Methods for immuno-depletion of specific factors from extracts and immunolocalization of specific proteins on chromosomes are also presented. An excellent compilation of detailed discussions and methods for the study of nuclear and cell cycle dynamics using Xenopus oocyte and egg extracts has been compiled by Kay and Peng (1)
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