Controllable Construction of Micro/nanostructured NiO in Confined Microchannels via Microfluidic Chemical Fabrication for Highly Efficient and Specific Absorption of Abundant Proteins

摘要: Three kinds of micro/nanostructured NiO arrays were constructed in confined microchannels via a facile and template-free microfluidic chemical fabrication method. Bovine serum albumin (BSA) and bovine hemoglobin (BHb) with different isoelectric points (IEPs) were chosen as the model proteins to test the absorption ability of NiO-modified microchannels for abundant proteins via electrostatic interaction and affinity interaction. The influences of the pH and ionic strength of the protein solution, the residence time of protein solution in the microchannels, zeta potentials and morphologies of nickel oxide on the protein absorption behavior of the modified microchannels were all studied. The NiO nanosheet array-modified microchannels could almost absorb all of the target proteins when the protein solution (500 μg mL−1) resided in the microchannel for 120 s without separation. The excellent protein absorption ability …