An Improved Method for the Measurement of Tenocyte Proliferation In-Situ

摘要: In-vitro studies of tenocytes aim to understand the relationship between the cells and their surrounding matrix [1], and how this interaction is affected by external stimuli placed upon them such as mechanical forces. For this reason it is important to understand whether cell proliferation is altered by mechanical or other stimuli. Currently the most commonly used methods of measuring cell proliferation rely on the use of BrdU (5-bromo-2’-deoxyuridine) or PCNA (proliferating cell nuclear antigen), which are visualised using antibody staining, therefore, relying on the permeabilisation of the cell membrane to detect what stage of the cell cycle the cells are in. EdU (5-ethynyl-2’-deoxyuridine) is a novel alternative to the BrdU assay for the measurement of active DNA synthesis. EdU is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis [2]. Detection of EdU is based on …