Isolation and identification of an in vivo reaction product of 6-hydroxydopamine

摘要: The product of oxidized 6-OHDA and GSH reacted in vitro has been identified by a variety of chemical and physical methods to be 2, 4, 5-trihydroxy-6-S-(glutathionyl) phenethylamine. Its chemical properties show it easily undergoes a variety of oxidative condensations and polymerization. Its oxidized form, the p-quinone, can be identified in small quantities in rat brain and mouse brain 1-3 h after 6-OHDA injection. This is believed to be the first report of a chemically identified species resulting from the in vivo interaction of 6-OHDA with CNS tissue.The molecular mechanism whereby 6-hydroxydopamine (6-OHDA) exerts its powerful neurotoxicity toward catecholamine neurons is still in question. However, from the beginning it has been suggested that nucleophilic interaction (covalent bonding) of the 6-OHDA quinone with neuronal constituents might be crucial in this action. 14 This view has receivedrecent support …