Abstract P061: Effect Of Short Chain Fatty Acid Butyrate On Cardiovascular Parameters In The Zebrafish Larvae

摘要: Introduction: Short-chain fatty acids (SCFAs) such as butyrate are produced by microbial fermentation of dietary fiber in the gut. While the effects of butyrate on cardiovascular function have previously been described in rodent models, no studies to date have investigated butyrate effects on cardiovascular function in zebrafish (ZF). ZF have become a widely used including in cardiovascular and gut dysbiosis research, and bacterial communities from adult ZF intestines can reportedly synthesize butyrate. Here, we investigated chronotropic and vasoactive effects of acute administration of butyrate in ZF larvae. Methods: ZF larvae (7-9 dpf) were anaesthetized with tricaine by immersion (MS-222, 0.3mM in larvae water). For measurements of heart rate (HR), either butyrate (40mM final concentration, ph=7.5) or vehicle (larvae water) was added to the ZF chambers, and HR was recorded with phase contrast microscopy …