Effects of hypoxen on activities of Na, K-ATPase, glycolytic and antioxidant enzymes in human erythrocytes

摘要: Hypoxen is clinically effective in surgical treatment of benign prostatic hyperplasia and a number of other pathologies. However, molecular mechanisms underlying the effects of hypoxen have not been clearly identified. This study was designed to examine if hypoxen affects Na+, K+-ATPase, glycolytic and antioxidant enzyme activities in human erythrocytes. Cells were treated with different concentrations of hypoxen and then enzyme activities were determinated spectrophotometrically. Hypoxen at 56 µM did not change Na+, K+-ATPase, hexokinase, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase activities and at 1.4–14 µM, activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. 28 and 56 µM hypoxen increased superoxide dismutase activity by 66% and 94%(p< 0.001), decreased glutathione peroxidase activity by 17% and 21%(p< 0.02), respectively, and had no effect on catalase and glutathione reductase activities. These effects of hypoxen can contribute to post-operative wound healing at a faster rate, to postoperative improvements in clinical and laboratory features, and to a decrease in the risk of postoperative complications.