Adaptation of Saccharomyces cerevisiae to growth with benomyl involves the strong YAP1-dependent transcriptional activation of FLR1 gene, encoding a plasma membrane MFS-MDR transporter

摘要: Following widespread use of the systemic fungicide benomyl in agriculture and horticulture, benomyl resistant strains of many fungal pathogens have emerged. Saccharomyces cerevisiae FLR1 gene encodes a transporter of the Major Facilitator Superfamily (MFS) conferring resistance to multiple drugs, in particular to benomyl 1. Fluorescence microscopy observation of distribution of the Flr1-GFP fusion protein in yeast living cells indicates that Flr1p is in the plasma membrane. The adaptation of S. cerevisiae to growth in the presence of the benomyl involves the dramatic activation (approximately 400 fold) of FLR1 transcription. This activation correlates to the content of Flr1p in the plasma membrane, as shown by quantitative immunodetection. This activation takes place during benomyl-induced latency following sudden exposure of the unadapted yeast population to this antimitotic fungicide and is completely …