Water intake depletes concentration of extracellular vesicles in peripheral blood

摘要: Background Extracellular vesicles (EVs) have been identified as promising in diagnosis and treatment of different diseases and in assessment of the state of the organism. The advantage of EV-based methods is that EVs can be isolated from body fluids, which are obtained by minimally invasive procedures. However, methods for EV harvesting and assessment have not yet reached sufficient repeatability and accuracy to be introduced in clinical practice. Also it is not yet clear how a person should be prepared for sampling of the body fluid. Here we considered the effect of water intake before the sampling on the concentration of EVs in isolates from peripheral blood. Methods Twenty-six healthy human adult volunteers (average age 39.8 years) were included in the study. After recording the drinking habits, the intake of water was increased for 30% before the first blood sampling and then decreased for 30% before …