Oxidative Stress and Inflammatory Responses in an Induced Subacute Acidosis Challenge

摘要: We examined the effects of grain, fructose and histidine on oxidative stress and inflammatory response in dairy cattle during an induced subacute acidosis (SARA) protocol. Thirty Holstein-Friesian heifers were randomly allocated into 5 groups; 1. Control (no grain), 2. Grain (1.2% liveweight (LW) rolled triticale)(GR), 3. Grain (0.8% LW)+ fructose (0.4% LW)(FR), 4. GR+ histidine (6g/head)(GR+ HIS) and 5. FR+ histidine (FR+ HIS). Rumen samples were collected 5, 65, 115, 165 and 215 minutes after challenge and analysed for histamine concentration. Plasma samples taken 5 and 215 minutes after challenge were analysed for histamine, advanced oxidation protein products (AOPP), glutathione peroxidase activity, ceruloplasmin, biological antioxidant potential (BAP) and reactive oxygen metabolites (dROMS). The effect of group and the interaction between group and time were analysed by repeated measures ANOVA and were not significant for any variables, with the exception of a plasma histamine group by time interaction. Ceruloplasmin concentrations were greater (P< 0.001) at 5 versus 215 minutes after challenge (0.19±0.07 and 0.09±0.01 g/L respectively). Concentrations of dROMs (P= 0.002) and the ratio of dROMs to BAP (P= 0.009) were higher at 215 minutes (123.5±3.3 U/dL and 0.041±0.002 respectively), compared to concentrations 5 minutes after challenge (108.7±4.1 U/dL and 0.048±0.003 respectively). No correlation between ruminal and plasma histamine concentrations (R=-0.141) was observed. In summary, the SARA protocol may not have provided sufficient challenge to produce a marked oxidative stress or an inflammatory …