T use of ELISA (enzyme-linked immunosorbent assay) has been demonstrated over the past 15 years (1, 2). In

摘要: * Author to whom correspondence should be addressed. many cases, ELISA has proven to be a sensitive and selective analytical method. Although some immunoassays can bc very selective, a drawback can be that antibodies cross-react with structurally related compounds; therefore, the signal obtained in an ELISA may not strictly relate to only one compound. This is particularly the case in environmental analysis, where a num-ber of structurally related compounds could be present in the matrix. Multianalyte analysis, therefore, could be done by combining different antibodies, as shown by Muldoon et al.(3) for the analysis of s-triazines, or by using one antiserum of different bleeds combined with LC (liquid chromatography), as shown for the screening off-agonists (4). Astep further in this direction is the integration of ELISA with chromatographic procedures, which is demonstrated in this paper. LC is an analytical …