Effects of bone marrow-derived mesenchymal stem cells on airway remodeling in a mice ovalbumin-induced asthma model

摘要: INTRODUCTION: ASTHMA IS A CHRONIC RESPIRATORY DISEASE CHARACTERIZED BY AIRWAY INFLAMMATION, AIRWAY HYPER RESPONSIVENESS AND MUCUS HYPER SECRETION. CHRONIC INFLAMMATION CAUSES TISSUE INJURY AND SUBSEQUENT STRUCTURAL CHANGES OF THE AIRWAYS, INCLUDING SUB EPITHELIAL FIBROSIS, GOBLET CELL HYPERPLASIA, AIRWAY SMOOTH MUSCLE HYPERTROPHY AND ANGIOGENESIS, COLLECTIVELY KNOWN AS AIRWAY REMODELING. CURRENT ASTHMA THERAPIES HAVE THE ABILITY TO ATTENUATE THE AIRWAY INFLAMMATION BUT DO NOT HAVE THE CAPACITY TO REDUCE AIRWAY REMODELING. THEREFORE, IT IS CRUCIAL TO EXPLORE NEW THERAPEUTIC APPROACHES THAT CAN REDUCE AIRWAY REMODELING. BONE MARROW-DERIVED MESENCHYMAL STEM CELLS (BMSCS) ARE THE MULTI-POTENT PROGENITOR CELLS THAT REGULATE IMMUNE AND INFLAMMATORY RESPONSES. FURTHERMORE, THE BENEFICIAL EFFECTS OF BMSCS IN REDUCING REMODELING HAVE BEEN OBSERVED IN MANY DISORDERS SUCH AS LIVER, LUNG, KIDNEY AND HEART FIBROSIS. THEREFORE, IN THIS STUDY, WE ASSESSED EFFECTS OF BMSCS ON AIRWAY REMODELING IN AN OVALBUMIN-INDUCED ASTHMA MOUSE MODEL. METHODS: BALB/C MICE WERE DIVIDED INTO FOUR GROUPS: CONTROL GROUP, ASTHMA GROUP, ASTHMA+ PBS GROUP AND ASTHMA+ BMSCS GROUP. AIRWAYS HISTOPATHOLOGICAL CHANGES (INFLAMMATION, GOBLET CELL HYPERPLASIA AND SUBEPITHELIAL …