Triacylglyceride profiles of integumentary lipids isolated from three bat species determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

摘要: Lipids secreted from sebaceous glands in bat integument may play a role in determining host pathogenicity by the fungus Geomyces destructans in White-Nose Syndrome (WNS). To investigate this, I have developed analytical methods to determine the triacylglycerol (TAG) profiles for three bat species: Eastern red bats (Lasiurus borealis, Müller, 1776), evening bats (Nycticeius humeralis, Rafinesque, 1818), and big brown bats (Eptesicus fuscus, Beauvois, 1796). Neutral lipids extracted from the hair and wing tissue were fractionated by preparative thin-layer chromatography (TLC) into four major lipid bands corresponding to cholesterol, free fatty acids (FFAs), TAGs, and sterol/wax esters. Densitometry showed higher proportions of TAGs in hair than from wing tissue in all four species. TAG bands were recovered and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI …