A new modified nutrient media for Cultivation of Actinobacteria

摘要: The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces A11, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2 …