Induction of defense mechanisms in coffee (Coffea arabica L.) against coffee leaf rust caused by Hemileia vastatrix by native rhizobacterial isolates.

摘要: Soil application of microbial consortium comprising Azospirillum ASW13, Azotobacter AZK 9, Gluconeobacter GDT13, phosphobacteria PBY23, Pseudomonas fluorescens PKK 9 and arbuscular mycorrhizal fungi (Glomus fasciculatum and Glomus spp.) were used to induce resistance in coffee against H. vastatrix. Other treatments included foliar and soil application of P. fluorescens PFK9 and Pf1. The experiment was conducted in Tamil Nadu, India during 2005-06. The induced systemic resistance was assessed by observing the activities of enzymes (peroxidase, polyphenol oxidase [catechol oxidase] and phenylalanine ammonia-lyase) and the contents of defence-inducing chemicals like total phenols. Microbial consortium and Pseudomonas were supplied at 20 g/plant, while mycorrhizas were supplied at 10 g/plant. Leaves were collected at 0 and 12 h; and 1, 3, 4, 14 and 21 days after the second …