Enhanced tissue adhesiveness of injectable gelatin-based hydrogels using thiomer

摘要: Tissue adhesives have received much attention as alternative materials to medical sutures and staples. Although they have been widely used for the wound closure and management, their adhesive strength and biocompatible properties are still need to be improved for variously clinical applications. Thiomers are promising candidates for adhesive improvement since thiol groups can react with cysteinerich glycoproteins on tissue to form disulfide crosslinked networks. We thus hypothesized that adhesive strength of 3 (4 hydroxyphenyl) propionic acidgelatin (GH) hydrogels formed by horseradish peroxidase (HRP) catalyzed oxidation can be improved with the addition of thiolated gelatin (GS). Furthermore, HRP not only catalyzes the oxidative coupling of phenols but also induces the disulfide formation in the presence of HO or under aerobic condition. This combination leads to form synergistic networks of phenolphenol and disulfide bonds for increasing an intragel crosslink and extra geltissue interactions (Fig. 1).