RAPD markers linked to rice blast (Magnaporthe grisea cav.) resistant gene

摘要: A miippmgpopulationwascomtituted by crossing an indica rice cultivarflR 5690) with durable resistance to blast disease with highly susceptible indica rice cultivar IR 50 and the F9 recombinant inbred lines (RILs) were produced by single seed descent method. The mapping population along with parents were evaluated for blast resistance in the glass house and in the field and categorized based on the reaction scores. In the parental sun-ey, the resistant and susceptible parental lines (IR 5690 and IR 50) were analysed with 65 random amplified polymorphic DNA primers (RAPD). Subsequently resistant and susceptible bulks were constituted and Bulk Segregant Analysis (BSA) was done with 49 RAPD primers, which showed polymorphism in parental survey. Particular fragments of five primers OPAH 18, OPU 15 and OPC14, OPK17, OPK11 were found to be co-segregated with resistant and susceptible phenotypes respectively in BSA. Results on individual RILs survey with the RAPD.. primer OPAH I8m showed a perfect cosegregation pattern with resistant phenotype. This could be used as a molecular marker for identifying resistant phenotypes in a segregating population involving susceptible and resistant blast varieties.