Analysis of Ptprv (OST-PTP/Esp) in adipose tissue and adipocyte differentiation

摘要: Ptprv is a transmembrane tyrosine phosphatase expressed in embryonic stem cells, gonads, and osteoblast precursors. In bone formation, it has been characterized as a molecular marker of mesenchymal bone collar cells and shown to play a role in osteoblast differentiation. We show for the first time that Ptprv is also expressed in adipose tissue. In this tissue, Ptprv expression is restricted to cells of the stroma vascular fraction, which contain adipocyte precursors, but is not detected in differentiated adipocytes. Moreover, Ptprv expression is regulated. Indeed, both in 3T3L1 cells and mouse embryo fibroblast models of adipocyte differentiation, Ptprv expression increases when cells reach confluence and then decreases upon exposure to adipogenic conditions. However, Ptprv expression patterns do not strictly overlap with Pref-1 and ACLP, two proteins expressed in preadipocytes but not in adipocytes. In addition …