Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

摘要: Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune cell functions in vitro and in vivo. These include: migration and adhesion; proliferation of stem and progenitor cells; differentiation and growth control; mechanisms of antigen presentation (eg, membrane transfer, phagocytosis); and interactions of effector and regulatory cells with each other and with tumor cells. This article summarizes methods for optimal staining with these dyes and provides examples from the recent literature illustrating their use to study mechanisms involved in tumor progression and anti-tumor immunotherapy.