Investigation on the Alcian Blue-tetrakis (methylpyridinium) chloride reactivity in presence of DNA, RNA, G-quadruplex structures and BSA

摘要: Phtalocyanines have the potential to act as anticancer drugs, thanks to their interactions with proteins and polynucleotides [1]. Their strong and specific interaction with G-quadruplex structures may be responsible for inhibiting cancer cells proliferation [2]. Phtalocyanines are excellent macrocyclic ligands for transition metals and metal coordination deeply modulates their chemical and photophysical properties.We focused our attention on Alcian Blue-tetrakis (methylpyridinium) chloride (ABTP), a commercially-available Cu (II)-phtalocyanine complex with promising features. The binding properties in presence of several polynucleotides and a protein were investigated at physiological conditions through spectrophotometric and spectrofluorometric techniques, thermal denaturation tests and calorimetric measurements. The measurements were repeated at different temperatures and ionic strengths in order to obtain information on the binding modes. The thermodynamic parameters were evaluated as well.