作者： MC Castrovilli , J Chiarinelli , P Bolognesi , E Tempesta , P Calandra
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摘要: Immobilization of enzymes on the surface of transduction systems represents a compulsory and critical phase in the development of biosensors. Indeed, by influencing orientation, loading, and mobility of enzymes by means of tailored immobilisation procedures, it is possible to tune their structure and biological activity for achieving enhanced analytical performances in terms of sensitivity, selectivity, and stability. In the specific case of the laccase enzyme, this concern was elegantly dealt by Rodríguez-Delgado and co-workers in their review asserting that “to become viable industrial catalysts, laccases need to be subject to treatments in order to make them robust, recyclable, or heterogeneous. One of the most studied treatments is immobilization, defined as attachment of an enzyme to an insoluble support. The benefits of an efficient protocol of immobilization are very important, namely prolonged use of the sensor …