Compounds and methods for nucleic acid mismatch detection

摘要: BACKGROUNDDNA base-pair mismatches arise during the course of genetic recombination and replication as a consequence of enzymatic errors or DNA damage. In the cell, there exist systems exist capable of recognizing and correcting these mistakes. In certain diseases, particularly cancer, these repair systems fail and mismatches persist in a diseased cell’s DNA. Therefore, providing compounds and methods designed to recognize site speci? c mismatches in DNA is important for genetic screening and for the design of neW chemotherapeu tics.Because many human diseases arise by single base pair changes in genes, the analysis of base pair mismatches and mutation has important implications in biomedical research and in medicine. For example, a considerable number of human genetic diseases are knoWn to be caused by point mutation.