Unique genetic architecture of prolificacy in ‘Sikkim Primitive’maize unraveled through whole-genome resequencing-based DNA polymorphism

摘要: ‘Sikkim Primitive’is a native landrace of North Eastern Himalayas, and is characterized by having 7–9 ears per plant compared to 1–2 ears in normal maize. Though ‘Sikkim Primitive’was identified in the 1960s, it has not been characterized at a whole-genome scale. Here, we sequenced the entire genome of an inbred (MGUSP101) derived from ‘Sikkim Primitive’along with three non-prolific (HKI1128, UMI1200, and HKI1105) and three prolific (CM150Q, CM151Q and HKI323) inbreds. A total of 942,417 SNPs, 24,160 insertions, and 27,600 deletions were identified in ‘Sikkim Primitive’. The gene-specific functional mutations in ‘Sikkim Primitive’were classified as 10,847 missense (54.36%), 402 non-sense (2.015%), and 8,705 silent (43.625%) mutations. The number of transitions and transversions specific to ‘Sikkim Primitive’were 666,021 and 279,950, respectively. Among all base changes,(G to A) was the most …