作者: Maxim G Brevnov , Hemant S Pawar , Manohar R Furtado , Jaiprakash Shewale
DOI:
关键词:
摘要: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention pro vides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of deter gents and solvent. When the particles are magnetically sepa rated out of the solution, the nucleic acids are separated with them. The nucleic acids can Subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in down stream molecular techniques such as quantification, identifi cation of the source of the nucleic acids, and genotyping.