118.6: The role of gastrin in human islet and type 1 diabetes animal model

摘要: Methods: In vitro culture: Isolated human islets were cultured (100 IEQ/per-well) in 6-well trans-well plates in a hyperoxia culture system under 50% oxygen for two weeks, and with or without 100 nM gastrin. Islet morphology, function and cell viability were assessed post-culture. Islet mRNA expression was determined by RT-PCR, and secretion of pro-inflammatory cytokines were detected using Luminex assay. Western blot was used to determine if reduction in NFkb pathway is involved in gastrin pro-survival effects on islets.In vivo animal study: 7-week-old NOD mice were injected ip with and without gastrin at 600 mg/kg daily for 14 weeks. Mice were monitored for blood glucose levels weekly. At the end of the 14-week injections, the intraperitoneal glucose tolerance test (IPGTT) was performed. The pancreas tissues were collected and sectioned for H&E staining to assess insulitis, and stained with anti-insulin, and …