Research Article Prediction and In Silico Identification of Novel B-Cells and T-Cells Epitopes in the S1-Spike Glycoprotein of M41 and CR88 (793/B) Infectious Bronchitis Virus Serotypes for Application in Peptide Vaccines

摘要: Bioinformatic analysis was used to predict antigenic B-cell and T-cell epitopes within the S1 glycoprotein of M 1 and CR88 IBV strains. A conserved linear B-cell epitope peptide, YTSNETTDVTS 175–185, was identified in M 1 IBV strains while three such epitopes types namely, VSNASPNSGGVD 279–290, HPKCNFRPENI 328–338, and NETNNAGSVSDCTAGT 54–69, were predicted in CR88 IBV strains. Analysis of MHCI binding peptides in M 1 IBV strains revealed the presence of 15 antigenic peptides out of which 12 were highly conserved in 96–100% of the total M 1 strains analysed. Interestingly three of these peptides, GGPITYKVM 208, WFNSLSVSI 356, and YLADAGLAI 472, relatively had high antigenicity index (> 1.0). On the other hand, 11 MHCI binding epitope peptides were identified in CR88 IBV strains. Of these, five peptides were found to be highly conserved with a range between 90% and 97%. However, WFNSLSVSL 358, SYNISAASV 88, and YNISAASVA 89 peptides comparably showed high antigenicity scores (> 1.0). Combination of antigenic B-cells and T-cells peptides that are conserved across many strains as approach to evoke humoral and CTL immune response will potentially lead to a broad-based vaccine that could reduce the challenges in using live attenuated vaccine technology in the control of IBV infection in poultry.