Diagnostic value of housekeeping [glmM] gene expression in antral biopsies in comparison to rapid urease test and histological detection of Helicobacter Pylori infection

摘要: Helicobacter pylori (H. pylori), a common infectious bacterium, has been linked to chronic gastritis, peptic ulcer and gastric cancer. Several invasive and noninvasive techniques are currently used for detecting H. pylori infection. Molecular approaches based on DNA amplification by polymerase chain reaction (PCR) have been developed for the detection of H. pylori in clinical samples but are used only in the research setting. Our objective is to comparatively evaluate the efficiency of using PCR technique for diagnosis of H. pylori infection through determination of glmM gene, and the rapid urease test (RUT), with reference to histological detection of H. pylori as the gold standard. Two hundred gastric biopsy specimens from the antrum of 50 dyspeptic patients were subjected to histology, RUT and PCR; targeting the glmM gene. No age or gender difference regarding H. pylori infection was observed, H. pylori colonization was detected in 82% of cases. The sensitivity and specificity of the RUT and PCR for glmM methods in comparison to histology are as follows: 87.80% and 88.89%; 65.9% and 44.4%, respectively. Histology demonstrating the highest detection rate among cases with superficial gastritis and gastroduodenitis (82% and 80%) compared to RUT (65.5% and 70%) and glmM gene that was expressed in 41.4% and 40% respectively. Colonization of the gastric mucosa by H. pylori was detected in 61.9% of the patients with chronic superficial gastritis, While, RUT was positive in 38.1% of them. Nearly the same percentages were observed for histology and RUT in the other histopathological findings. Similar percentage for glmM gene as …