EXPRESSION AND PURIFICATION OF RHBMP-7 (RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-7) IN E. COLI USING SUMO FUSION PARTNER

摘要: BACKGROUND AND AIM: BMPS ARE THE MEMBERS OF TRANSFORMING GROWTH FACTOR Β (TGF-Β) SUPER FAMILY, AND PLAY AN IMPORTANT ROLE IN BONE METABOLISM. RECOMBINANT FORMS OF BMP-2 AND BMP-7 ARE THE ONLY BMPS USED CLINICALLY. BONE MORPHOGENETIC PROTEIN-7 (BMP-7) IS A GROWTH FACTOR PREDOMINANTLY RECOGNIZED FOR ITS OSTEOINDUCTIVE PROPERTIES. DUE TO THE HIGH COST OF THIS PROTEIN, THE HETEROLOGOUS PRODUCTION OF THIS PROTEIN HAS BEEN PERFORMED IN A NUMBER OF EXPRESSION SYSTEMS SUCH AS ESCHERICHIA COLI. UNFORTUNATELY, MOST RECOMBINANT PROTEINS ARE EXPRESSED AS INCLUSION BODIES IN E. COLI. IN THIS WORK, SUMO FUSION SYSTEM WAS USED IN ORDER TO IMPROVE THE SOLUBILITY AND EXPRESSION OF RECOMBINANT HUMAN BMP-7 (RHBMP-7) IN ESCHERICHIA COLI. SMALL UBIQUITIN-RELATED MODIFIER (SUMO) IS AN ACIDIC FUSION TAG THAT COVALENTLY LINKS TO TARGET PROTEINS. SUMO FUSION SYSTEM HAS BEEN SHOWN TO ENHANCE PROTEIN EXPRESSION AND SOLUBILITY AND ALSO INCREASE PROTEIN FOLDING VIA CHAPERONING PROPERTIES....