Detection of blaCTX, blaSHV and AAC-3-IV resistance genes among selected Klebsiella sp, Escherichia coli and Proteus mirabilis isolated from feacal droppings of Meleagris gallopavo f. domestica

摘要: The study reported the detection of resistance genes among selected isolates from feacal droppings of Meleagris gallopavo in Ile-Ife, Nigeria; with the view to determining their antibiotic susceptibility profile and nature of resistance genes. Faecal droppings of turkey were collected at random from various poultries into sterile universal bottles and were cultured on MacConkey agar using pour plate technique. Antibiotic susceptibility of isolates was examined and detection of resistance genes was carried out by Polymerase Chain Reaction (PCR) using appropriate primers. Eight of the 258 samples of faecal droppings of turkey were devoid of any visible bacterial growth. Bacterial isolates identified include Klebsiella sp.(26%), Escherichia coli (22%), Proteus sp (8%), Aeromonas (8%), Enterobacter sp.(8%), Citrobacter sp.(7%), Serratia sp.(7%), Yersinia sp.(6.1%), Shigella sp.(4.1%), and Providencia sp.(3.3%). Resistance to antibiotic varied among the isolates. 32% of the isolates was resistant to ceftazidime,(43%) to cefuroxime, gentamycin (23%), 42% to cefixime, and 100% to Augmentin. The isolates were 94% susceptible to nitrofurantoin. Three of the twelve selected MAR bacterial harboured bla-CTX-M gene, K. ornithinolytica harboured AAC-3-IV gene. The study concluded that poultry litters could serve as an environmental reservoir of multiple antibiotic resistant bacteria. The detection of the resistance genes have great health and economic consequences. Therefore, effective hygiene of poultry environment should be emphasized. Treatment options for bacterial infections from this study include; nitrofurantoin, gentamicin, ofloxacin and …