Renin purification

摘要: The first complete purification of renin (EC 3.4.99.19) has only been achieved during this last decade. The instability of the unpurified enzyme and its extremely low concentration in kidney and biologic fluids explain the difficulties encountered during the course of the purification. The complete purification of renin is a mandatory step for our understanding of the renin-angiotensin system, allowing the production of highly specific renin antibodies and the study of the possible renin precursors, the active site of the enzyme, its structure, and the mechanisms of its inactivation.