Evaluation of Serial Measurements of Selected Biomarkers in Serum and Peritoneal Fluid for the Diagnosis of Intestinal Ischemia in Donkeys: An Experimental Study

摘要: Up to now, there has been limited evidence relating to the early diagnostic approach of colic in equine patients. The purpose of this study was to evaluate serial measurements of selected biomarkers in both serum and peritoneal fluid (PF) in donkeys underwent experimental intestinal obstruction owing to early detection of intestinal ischmia. For this purpose, five apparently healthy donkeys (Equus asinus) were randomly purchased based on their health condition and were thoroughly examined to rule out concurrent or even previous ailments. Following a standard pre-surgical and surgical precautions, these animals were subjected to intestinal ischemia via complete ligation of ileum and its mesentery. The animals were clinically and ultrasonographically monitored for 72 hours. Paired samples of blood and PF were collected from each donkey before surgery (T0) and following surgical intervention at the following time points: T12, T24, T48 and T72 to measure the respective levels of L-lactate, nitric oxide (NO), alkaline phosphatase (ALP), lipase enzyme and cardiac troponin I (CTnI). The results herein demonstrated that the pattern of L-lactate, ALP, lipase enzyme and CTnI in both PF and serum were significantly higher at both T48 and T72 compared to those at other time points (P< 0.05). However, the pattern of NO in both PF and serum showed a significant (P< 0.05) higher values at T12, T24, T48 and T72 when compared with T0. The obtained findings indicated that both PF and serum can be used as a potential diagnostic indicator for the early detection of intestinal ischemia in donkeys.