Detection of DNA mismatches and oxidative lesions

摘要: The present invention describes methods for directly label ing the 3'-phosphate end at a nucleotide Site. Further, as internal 3'-phosphate termini on DNA duplexes are also asSociated generally with Oxidative lesions, these methods provide a general Strategy for labeling, and therefore, detect ing the frequency of oxidative DNA lesions. The present invention also discloses labeling methods using terminal transferase or nontemplated DNA polymerization, where the use of either of these activities affords tagging a Site, after removal of the 3'-phosphate, with polynucleotide tails. Such polynucleotide tails in turn can function as primer binding Sites for use in PCR in gene analyses.