Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

摘要: Multiple sensitive eco‐friendly analytical techniques were applied for the assessment of two important fourth‐generation fluoroquinolones namely, gemifloxacin (GFX), and gatifloxacin (GTF). GFX, which is utilized as an essential adjunct therapy for the management of pneumonia in SARS‐CoV‐2 was determined by green indicating micellar liquid chromatographic in authentic powder and dosage forms. The separation was performed by using a reversed‐phase C18 column; the micellar mobile phase comprised of 12.5% n‐propanol, 0.15 M sodium lauryl sulfate, and 0.3% triethylamine in 0.02 M O‐phosphoric acid adjusted to pH 3.0 and pumped at 1 ml/min flow rate and detected at 266 nm by UV detection. The method was linear over the range of 4.0–90 μg.ml−1 with a recovery percentage of 99.81 ± 0.69. The acidic degradation behavior of GFX along with the kinetic investigation was evaluated as …