Development of an in vitro blood-brain barrier model for brain disposition screening of pharmaceuticals

摘要: Solute distribution between blood and brain is strictly regulated by the blood-brain barrier (BBB). While the BBB performs an important function in keeping unnecessary or harmful molecules from the brain, it poses a challenging problem in delivering therapeutics, including anticancer, antibiotic or antipsychotic drugs into the brain. Conversely, preventing potentially damaging molecules from overcoming the BBB is also an increasing problem, especially when combinations of therapeutics are encountered. Medical and pharmaceutical scientists therefore have a growing need for rapid, reliable in vitro models of the BBB for preclinical screening of pharmaceutical BBB transport properties. The current presentation describes initial results in development of an in vitro BBB model derived from bovine brain capillary endothelial cells (BCEC). Capillary vessels were first isolated from bovine brains. Individual BCECs were …