Overturning the Paradigm of miRNA Regulation: Upregulation as a common mechanism of miRNA action

摘要: DiscoverBMB Abstracts Abstract 1896 Regulation of senescent cell survival by a GBA pseudogene Igor Ulitsky, Weizmann Institute of Science Filipa Carvalhal Marques, Noa Balaban Selective clearance of senescent cells in vivo has been shown to delay emergence of aging-associated diseases and to lengthen lifespan. The metabolic processes occurring within senescent cells are key to their special properties. Here, we used CRISPRbased screening to identify and characterize a long noncoding RNAs (lncRNAs) that support survival of senescent cells through metabolic regulation. In order to study survival of senescent cells, we used the DNA damage response of BJ fibroblasts paradigm and conducted a CRISPRi-based loss-offunction screen targeting all lncRNAs expressed before and after cells enter senescence. Guides depleted in cells 10 days after senescent entry were used to identify lncRNAs required …