Sulfonation and anticoagulant activity of botryosphaeran from< i> Botryosphaeria rhodina</i> MAMB-05 grown on fructose

摘要: Botryosphaeran (EPSFRU), an exopolysaccharide of the β-(1→3,1→6)-d-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. Botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. The effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and FT-IR analysis of the sulfonated EPSFRU (once sulfonated, EPSFRUSULF; and re-sulfonated, EPSFRURESULF). Activated partial thromboplastin time (APTT) and thrombin time (TT) tests of EPSFRURESULF indicated significant in vitro anticoagulant activity that was dose-dependent. EPSFRU did not inhibit any of the coagulation tests.