FERRITIN SYNTHESIS AND TURNOVER IN IRON STIMULATED RAT SKELETAL MYOBLASTS.

摘要: Ferritin synthesis and turnover were studied in the rat skeletal muscle derived cell line, L-6, as a means to further our understanding of iron metabolism in muscle. An immunoradiometric assay was developed in order to quantitate ferritin in lysates of these cells: this assay was shown to be sensitive and specific, and to conform to the antibody excess conditions required of it.Using this assay, it was shown that the L-6 cells synthesize ferritin in response to extracellular iron. Iron as the nitrilotriacetate chelate was better able to enter the cells and to induce ferritin synthesis than ferric citrate, over a range of iron concentrations. Ferritin synthesis in response to ferric nitrilotriacetate (FeNTA) was rapid in the first 8-24 hours; a second, slower phase of synthesis continued for up to 7 days if iron stimulation was continued. This change in synthetic rate was shown not to be due to increased turnover at later times; conversely it …