PYRIDINE ALKALOID ISOLATED FROM AN ENDOPHYTIC FUNGUS OF THE BRAZILIAN MANGROVE

摘要: Mangrove is a unique environment whose plants, animals and microorganisms are adapted to extreme conditions [1]. Endophytic fungi live in plant tissues without causing any negative effects and they have the ability to synthesize a variety of bioactive secondary metabolites that often depends on the plant habitat [2]. This study reports the isolation, structural characterization and antimicrobial activity of a pyridine alkaloid (PA) produced by the endophytic fungus 99 (3) isolated from Laguncularia racemosa leaves, which was collected in the mangrove forest of Bertioga, São Paulo state (Brazil)[2]. The fermentation broth was extracted with ethyl acetate (EtOAc), with m= 238.0 mg of the crude extract. This extract was submitted to chromatography column using 10 g of Silica gel (Waters Sep-PakR vac 35 cc) and eluted with gradient of MeOH: EtOAc. Thin Layer Chromatography analysis, revealed with Dragendorff …