Isolation, characterization and taxonomy of streptomyces sp Isolated from Jordanian soils and antagonistic to Agrobacterium tumefaciens

摘要: Of 161 streptomyces isolates previously isolated from soils in the Jordan Valley, 32 were found to be active against Agrobacterium tumefaciens, the causal agent of crown gall disease. These represent 20% of the isolates that were active against Gram-positive and Gram-negative bacteria. The distribution of these isolates antagonistic to A. Tumefaciens was as follow: 38% grey, 34% red, 13% green, 9% white, yellow and violet each 3%. From those 38% producted melanin, 34% produced soluble pigments, 59% showed distinctive reverse side pigment, and 63%, 22% and 3% had spiral, flexuous, straight, and retinculumapertum sporophore, respectively. All utilized arabinose and fructose followed by sucrose [97%], rhamnose [81%], mannitol and xylose [78% each], inositol [63%] and raffinose [41%]. 29 had smooth surfaced spore, 1 hairy and 2 spiny. 26 of them were completely identified to species level. They were divided into 18 different species. The highest frequency was in Streptomyces doghestanicus [22%], S. Vinaceusdrapus [17%]. S. boltropensis [17%], S. resistomycificus [17%]