Ethylene signaling modulates air humidity responses in plants

摘要: Air humidity significantly impacts plant physiology. However, the upstream elements that mediate humidity sensing and adaptive responses in plants remain largely unexplored. In this study, we define high humidity‐induced cellular features of Arabidopsis plants and take a quantitative phosphoproteomics approach to obtain a high humidity‐responsive landscape of membrane proteins, which we reason are likely the early checkpoints of humidity signaling. We found that a brief high humidity exposure (i.e., 0.5 h) is sufficient to trigger extensive changes in membrane protein abundance and phosphorylation. Enrichment analysis of differentially regulated proteins reveals high humidity‐sensitive processes such as ‘transmembrane transport’, ‘response to abscisic acid’, and ‘stomatal movement’. We further performed a targeted screen of mutants, in which high humidity‐responsive pathways/proteins are disabled, to …