New Zealand rabbit model of cisplatin-induced acute kidney injury

摘要: OBJECTIVE:To establish a New Zealand rabbit model of acute kidney injury induced by cisplatin.METHODS:A total of 24 male New Zealand rabbits were randomly divided into control group, low-, medium-and high-dose cisplatin group according to the body mass. Rabbits were injected with cisplatin at 0. 0, 1. 0, 2. 0, 4. 0 mg/kg body weight by auricular vein. Rabbits in low-dose group was continuously injected for 5 days, medium-dose group was continuously injected for 3 days, and the high-dose group was injected for once per day. Rabbits in the control group did not receive any treatment. Blood was collected from the middle ear artery and 24 h urine was taken before exposure and on day 1, day 3, day 5 and day 7 of injection. The serum creatinine (Scr) and urea nitrogen (BUN) were detected by colorimetric method, and 24 h urine kidney injury molecule 1 (KIM-1) was measured by enzyme-linked immunosorbent assay. Plasma platinum, 24 h urinary platinum and renal platinum level were detected by inductively coupled plasma mass spectrometry. At the end of the experiment, rabbits were sacrificed and the left kidney was taken for histopathological examination.RESULTS:The body mass of rabbits of the low-, medium-and high-dose groups on day 7 after cisplatin exposure was lower than that of the control group (P< 0. 05), and lower than that of the same group before exposure (P< 0. 05). After 3 days of exposure, the Scr level in each dose group was higher than that of the control group (P< 0. 05), the Scr level on day 3and day 5 in medium-and high-dose groups were higher than that of the low-dose group (P< 0. 05). The BUN levels on day …