Transformation of lettuce with FMDV epitopes fused gene mediated by Agrobacterium

摘要: Plant-derived vaccine is becoming one focus of plant gene engineering, which has advantages such as easy production, low cost, convenient and safe use. In this experiment, the fused gene O 21-O 14-A 21 containing both type O and A FMDV epitopes was introduced into lettuce mediated by agrobacterium. The hygromycin-resistant lettuce calli were obtained by screening on the medium containing antibiotic. Fifty six hygromycin-resistant lettuce seedlings were regenerated. The results of PCR and PCR-Southern blotting indicated that the fusion gene were successfully integrated into the genomes some of transformed lettuce plants. Expression of O 21-O 14-A 21 gene in transformed lettuce plants was proved by RT-PCR (analyses).