Methods to Separate, Characterize, and Encapsulate Drug Molecules into Exosomes for Targeted Delivery and Treatment of Glioblastoma

摘要: There have been many drug delivery strategies for the effective targeted delivery of drugs across the blood-brain barrier (BBB) cells to overcome challenges posed by BBB. However, drug delivery to the brain is still a hurdle that has yet to be solved. Due to the tight junctions and high selectivity of the BBB, most active and passive strategies deliver an insufficient or insignificant amount of drug across the protective BBB shield. Recently, exosomes, “biological nanoparticles” with the inherent homing capability to brain cells, have been shown to deliver drugs efficiently by preserving their therapeutic activity. Many different drug molecules are loaded into exosomes, belonging to the category of small synthetic drug molecules (doxorubicin, rhodamine) or large protein-based molecules (catalase) or nucleic acid-based drugs such as small interfering RNA (siRNA). In this chapter, we will focus on describing exosome …