TRKA expression and NTRK1 gene copy number across solid tumors

摘要: Background: Neurotrophic Tropomyosin Kinase Receptor 1 (NTRK1) gene encodes for the protein Tropomyosin-related kinase A (TRKA). A deregulated activity of TRKA has been detected in cancer, leading to oncogenic activity. This can result from translocations, amplifications, deletions and point mutations involving the NTRK1 gene. The incidence of NTRK1 gene copy number gain (GCN) across solid tumors has not been investigated. We present here the results of an immunohistochemistry (IHC) screening for TRKA expression within the phase I ALKA-001 clinical trial. Clinical results of ALKA-001 clinical trial are not presented here.Methods: Formalin-fixed paraffin-embedded (FFPE) consecutive samples of different solid tumors were tested for TRKA IHC staining. Samples showing TRKA IHC staining in at least 10% of cells were further studied by fluorescence in situ hybridization (FISH) to assess whether …