Antioxidant and Anti-inflammatory Activities of Arundina graminifolia Leaf Extract

摘要: Arundina graminifolia (D. Don) Hochr. has been used as a traditional medicine for detoxification, anti-arthritis, and antidote for many years. This study aimed to determine the biological property of Arundina graminifolia leaf extract (ALE) for antioxidant and anti-inflammatory activities. Arundina graminifolia leaves were extracted with 70% ethanol, and antioxidant activity was evaluated by DPPH and ABTS assay. The iron-binding capacity of ALE was measured by spectrophotometric technique. Lipid peroxidation assay was determined using the thiocyanate method. Moreover, the production of TNF-α and IL-6 was measured by an ELISA kit. Our results showed that ALE containing total phenolic and flavonoid contents exhibited antioxidant activity by scavenging free radicals. The IC 50 values of ALE by DPPH and ABTS radical scavenging assay were 155.17±3.61 and 13.82±0.38 µg/mL, respectively. Furthermore, ALE could inhibit free radical production via the Fenton reaction by complex formation with Fe 3+-NTA. The scavenging activity of ALE in hemoglobin-induced lipid peroxidation resulted in 42% inhibition. Besides, ALE significantly inhibited LPS-induced pro-inflammatory mediators, including the production of NO and IL-6. These findings suggest that ALE could be used as a functional food or pharmaceutical product containing antioxidant and anti-inflammatory activities and providing therapeutic potential for treating inflammatory diseases.