Novel cell culture 3D model for HBV/HIV co-infection and antiviral evaluation

摘要: Results: In vitro analysis showed that all DNA-HBVac-encoded HBV proteins were properly expressed. Immunization of C57BL/6 mice with DNA-HBVac prime and MVA-HBVac boost proved to be safe and well tolerated. The regimen resulted in very strong HBV-specific CD8 and CD4 T-cell responses as compared to previous immunization strategies, but as expected low anti-HBs response. Employing persistent HBV infection model, AAV-HBV-transduced mice, we could demonstrate that priming with DNA-HBVac elicited strong core-, envelope-, and RT-specific CD8 T-cell response in the livers of the mice. Moreover, we have observed significant serum ALTelevation during DNA-HBVac vaccination, suggesting increased cytotoxic T cell activity in the liver. These vigorous HBV-specific CD8 T cell responses resulted in a significant serum HBsAg and HBeAg reduction and even HBV clearance in some mice …