Adenosine cyclic 3',5'-monophosphate and steroid production by small ovarian follicles from Booroola ewes with and without a fecundity gene.

摘要: The tissue contents of adenosine cyclic 3',5'-monophosphate (cAMP) in freshly dissected follicles (0.13-1.00 mm diam.) were significantly higher Booroola ewes containing a major fecundity gene (FF and F+ ewes) compared to those values Booroolas with no copy the (++ animals; P less than 0.025). After 1 h incubation LH + FSH, respective proportions diameter 0.13-0.52 (n = 288) 0.53-1.00 271) that had synthesized greater or equal 0.6 pmol cAMP 1.0 influenced by genotype (Booroola homozygous for F-gene, FF heterozygous, ++; 0.01 both follicle size ranges). progesterone, androstenedione, testosterone oestradiol-17 beta minced ethanolic extracts 188) undetectable regardless genotype. However, when but not cultured 48 FSH under 70 kPa 50% O2, 45% N2 5% CO2 gas mixture, high levels progesterone (greater 4.0 ng), androstenedione 3 oestradiol 0.8 ng) 0.05 each steroid). No significant genotypic differences noted synthesis. Collectively, these results show F-gene has an influence on maturation ovarian from early stage growth.