Detection of Oncogene Amplification by Fluorescence in Situ Hybridization (FISH)

摘要: The cytogenetic analysis of solid tumors by conventional karyotyping has been little studied because it requires the culture tumor cells and is very difficult to grow primary in vitro. Fluorescence situ hybridization (FISH), a powerful tool for genetic evaluation, permits microscopic identification localization aberrations not only metaphase preparations but interphase nuclei. In late 1980’s α-repetitive pericentromeric probes which specific individual chromosomes became available. This enabled detection chromosomal copy number abnormalities carcinomas bladder, breast, stomach, colon so on. FISH makes possible minor populations with abnormal numbers assessment intratumoral clonal heterogeneity numbers1–3). 1990’s, several gene have developed these probes, can localize on spreads, isolated nuclei or tissue sections directly. such cellular oncogenes as c-erbB-2, c-myc, N-myc abl, bcr are now commercially Although available at present limited, predicted that this will change decade. article reviews oncogene amplification detected mainly based my studies cmyc, c-met K-sam gastric cancer.