Development of a short-term reproductive endocrine bioassay using steroid hormone and vitellogenin end points in the estuarine mummichog (Fundulus heteroclitus)

摘要: We have developed a short-term gonadal recrudescence test with the estuarine mummichog (Fundulus heteroclitus) and determined endocrine end points sensitive to strong estrogen agonist (ethynylestradiol; EE2) an antiestrogen (ZM 189,154; ZM) at concentrations of 0 1,000 ng/L in three separate experiments. A protocol was ensure year-round supply recrudescing fish. for determining steroid production (testosterone 11-ketotestosterone [11-KT] incubated testes tissue testosterone 17-estradiol [E2] prematurational follicles) optimized. Recrudescing fish (males, gonadosomatic index = 2%; females 10%) were exposed graded doses EE2 or ZM 7 15 d using static daily-renewal protocol. At high (>250 ng/L), effect on males depression androgen steroidogenesis plasma levels. In females, depressed circulating E2 levels; however, 250 ng/L) increased 11-KT while decreasing (1,000 only). Male female vitellogenin responded concentration-dependent fashion no by ZM. The low observable parameters 1 bioassay results encompassing environmentally relevant exposure range (1–100 will be useful assessing effects endocrine-active contaminants environments.